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Validation
Studies
Correlation of
DAK-36 Results with HPLC Analysis
The Domoic Acid Screening Test Kit gives
equivalent results to those obtained using standard HPLC and FMOC-HPLC
methods. It has successfully
measured DA in phytoplankton and razor clams. (Figures 1 and 2
below) Correlation with HPLC was
observed over
a broad range of
concentrations in both
matrices. Standard extraction
methods were used prior to splitting the extracts for analysis by ELISA
and HPLC. Extracts containing high
concentrations of DA are determined by appropriate dilution to obtain a
relative signal in the ELISA assay between 15% and 85% of the control
signal.
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